Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Quercetin Inhibits the Proliferation and Metastasis of Human Non-Small Cell Lung Cancer Cell Line: The Key Role of Src-Mediated Fibroblast Growth Factor-Inducible 14 (Fn14)/Nuclear Factor kappa B (NF-κB) pathway

doi: 10.12659/MSM.920537

Figure Lengend Snippet: Administration of Que suppressed the proliferation potential of HCC827 cells. For MTT assays, cells were incubated with Que of 25 μM (Que L), 50 μM (Que M), and 100 μM (Que H) for 96 hours. Every 24 hours, cells were subjected to the testing. For colony formation assays, cells were cultured in medium containing 100 μM Que for 2 weeks. ( A ) Detection results of MTT assays. ( B ) Detection results of colony formation assays. * P <0.05 versus the Control group.

Article Snippet: Human NSCLC cell lines HCC827 (7-1150) and NCI-H1650 (7-1031) were obtained from Chi Scientific (MA, USA).

Techniques: Incubation, Cell Culture, Control

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Quercetin Inhibits the Proliferation and Metastasis of Human Non-Small Cell Lung Cancer Cell Line: The Key Role of Src-Mediated Fibroblast Growth Factor-Inducible 14 (Fn14)/Nuclear Factor kappa B (NF-κB) pathway

doi: 10.12659/MSM.920537

Figure Lengend Snippet: Administration of Que inhibited the invasion and migration potentials, and the signaling transduction of Src/Fn14/NF-κB pathway in HCC827 cells. For scratch assays, cells were incubated with Que of 100 μM (Que H) for 48 hours. For Transwell assays, cells were incubated with Que of 100 μM (Que H) for 24 hours. For western blotting assays, cells were incubated with Que of 100 μM (Que H) for 24 hours. ( A ) Detection results of scratch assays. ( B ) Detection results of Transwell assays. ( C ) Detection results of western blotting assays. * P <0.05 versus the Control group.

Article Snippet: Human NSCLC cell lines HCC827 (7-1150) and NCI-H1650 (7-1031) were obtained from Chi Scientific (MA, USA).

Techniques: Migration, Transduction, Incubation, Western Blot, Control

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Quercetin Inhibits the Proliferation and Metastasis of Human Non-Small Cell Lung Cancer Cell Line: The Key Role of Src-Mediated Fibroblast Growth Factor-Inducible 14 (Fn14)/Nuclear Factor kappa B (NF-κB) pathway

doi: 10.12659/MSM.920537

Figure Lengend Snippet: Overexpression of Src blocked the anti-NSCLC function of Que in HCC827 cells. Cells transfected with negative control vector (NC) or Src expression vector (Src) were treated with Que of 100 μM (Que H) and subjected to MTT assay for 96 hours (cells were collected every 24 hours), and colony formation was assay at 2 weeks, Transwell assay at 24 hours, and scratch assay at 48 hours. ( A ) Detection results of Src level. ( B ) Detection results of MTT assays. ( C ) Detection results of colony formation assay. ( D ) Detection results of Transwell assay. ( E ) Detection results of scratch assay. # P <0.05 versus Que H group.

Article Snippet: Human NSCLC cell lines HCC827 (7-1150) and NCI-H1650 (7-1031) were obtained from Chi Scientific (MA, USA).

Techniques: Over Expression, Transfection, Negative Control, Plasmid Preparation, Expressing, MTT Assay, Transwell Assay, Wound Healing Assay, Colony Assay

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Quercetin Inhibits the Proliferation and Metastasis of Human Non-Small Cell Lung Cancer Cell Line: The Key Role of Src-Mediated Fibroblast Growth Factor-Inducible 14 (Fn14)/Nuclear Factor kappa B (NF-κB) pathway

doi: 10.12659/MSM.920537

Figure Lengend Snippet: Que inhibited the growth and metastasis potential of NSCLC in vivo in by inhibiting Src signaling. Mice were injected with different HCC827 cells and administrated with Que of 100 mg/kg body weight for 3 weeks. ( A ) Detection results of tumor volume. ( B ) Detection results of hematoxylin and eosin detection of tumor tissue. ( C ) Detection results of western blotting detection of E-cadherin and N-cadherin. * P <0.05 versus Que H+Src group. Scale bar, 100 μm.

Article Snippet: Human NSCLC cell lines HCC827 (7-1150) and NCI-H1650 (7-1031) were obtained from Chi Scientific (MA, USA).

Techniques: In Vivo, Injection, Western Blot

Journal: Molecular Cancer Therapeutics

Article Title: Proanthocyanidins Inhibit In vitro and In vivo Growth of Human Non–Small Cell Lung Cancer Cells by Inhibiting the Prostaglandin E2 and Prostaglandin E2 Receptors

doi: 10.1158/1535-7163.mct-09-0638

Figure Lengend Snippet: Figure 1. In vitro treatment of human NSCLCs with GSPs inhibits the proliferation potential and induces apoptosis in a dose-dependent manner. A, comparative dose-dependent effect of GSPs on the proliferation potential of human NSCLC cells, A549, H1299, H226, H460, and H157, 48 h after treatment (29). B, the concentration- and time-dependent effect of GSPs on the proliferation potential of normal human bronchial epithelial cells (BEAS-2B). Cell viability was determined using the MTT assay as described in Materials and Methods and is expressed in terms of percent of vehicle-treated (0.2% DMSO) control cells; points, mean of eight replicates; bars, SD. C and D, GSPs induce apoptosis in A549 and H1299 cells. Apoptotic cell death was analyzed as detailed in Materials and Methods. C, the lower-right (LR) quadrant of the fluorescence-activated cell sorting histograms indicates the percentage of early apoptotic cells, and the upper-right (UR) quadrant indicates the percentage of late apoptotic cells. D, total percentages of apoptotic cells in A549 and H1299 are summarized; columns, mean from three experiments; bars, SEM. Significant difference versus non–GSP-treated controls: *, P < 0.05; ¶, P < 0.01; †, P < 0.001.

Article Snippet: The human NSCLC lines (A549, H1299, H226, H460, H1975, H1650, HCC827, and H157) were purchased from the American Type Culture Collection.

Techniques: In Vitro, Concentration Assay, MTT Assay, Control, Fluorescence, FACS

Journal: Molecular Cancer Therapeutics

Article Title: Proanthocyanidins Inhibit In vitro and In vivo Growth of Human Non–Small Cell Lung Cancer Cells by Inhibiting the Prostaglandin E2 and Prostaglandin E2 Receptors

doi: 10.1158/1535-7163.mct-09-0638

Figure Lengend Snippet: Figure 6. A, dietary GSPs inhibit the growth of A549 and H1299 non–small cell lung cancer cells (NSCLC) grown as xenografts in athymic nude mice (29). Tumor xenografts tissues were harvested at the termination of the experiment, and the wet weight of the tumor/mouse in each group is reported in grams as mean SD, n ¼ 10. Statistical significance versus non-GSP–treated controls, , P < 0.05; {, P < 0.01; †, P < 0.001. B, tumor xenograft tissues from control and GSP-treated (0.5%, w/w) mice were used for the analysis of the levels of COX-2 and PGE2 receptors using Western blotting. Dietary GSPs inhibit the levels of COX-2 and PGE2 receptors, EP1, EP3 and EP4, in the tumor xenograft tissues grown in athymic nude mice compared with control tumor xenograft tissues. Representative blots from A549 or H1299 xenografts are presented from theindependent analysis of tumors from 6 animals pergroup with identical results. The relative density (arbitrary) of each band after normalization for b-actin is shown under each immunoblot as the fold change compared with non-GSP–treated control, which was assigned an arbitrary unit 1 in each case. PGE2 was determined in tumor xenograft tissue samples using a PGE2 immunoassay kit following the manufacturer's instructions. The concentration of PGE2 is expressed in terms of pg/mg protein as a mean SD, n ¼ 10. Significantly lower versus non-GSP–treated controls. †, P < 0.001.

Article Snippet: The human NSCLC lines (A549, H1299, H226, H460, H1975, H1650, HCC827, and H157) were purchased from the American Type Culture Collection.

Techniques: Control, Western Blot, Concentration Assay

Journal: Oncology letters

Article Title: Isolation of circulating tumor cells and detection of EGFR mutations in patients with non-small-cell lung cancer.

doi: 10.3892/ol.2019.10016

Figure Lengend Snippet: Figure 3. Association of CTC counts among all patients based on tumor histology and staging. The mean number of detected CTCs is illustrated. (A) Patients with SCLC had the highest CTC counts and a CTC detection rate of 77.8% (7 of 9 patients; median 5; mean 8.22) compared with patients with NSCLC or other types of metastatic disease. (B) TNM Stage IV NSCLC was significantly associated with higher CTC numbers (Mann‑Whitney test, P=0.0081). At least 1 CTC was detected in 52 (69.3%) of the 75 patients with TNM stage IV disease. At least 1 CTC was detected in four (25.0%) of the 16 patients with TNM stage III disease. CTC, circulating tumor cell; SCLC, small‑cell lung cancer; NSCLC, non‑small‑cell lung cancer; SCC, squamous cell carcinoma; Adeno, adenocarcinoma; TNM, tumor, node, metastasis.

Article Snippet: A total of three commercially available lung cancer adenocarcinoma cell lines (NCI‐H2009, NCI‐H1975, and HCC‐827) were purchased from The American Type Culture Collection (Manassas, VA, USA) for spiking experiments and mutation analyses.

Techniques:

Journal: The Journal of Biological Chemistry

Article Title: Rho family small GTPase Rif regulates Wnt5a-Ror1-Dvl2 signaling and promotes lung adenocarcinoma progression

doi: 10.1016/j.jbc.2023.105248

Figure Lengend Snippet: Ror1 and Rif are colocalized at filopodia in LUAD cells. A, Western blot analysis of Ror1, Ror2, and Rif in LUAD cell lines, showing expression of both Ror1 and Rif were detectable in all LUAD cell lines examined. Blots are representative of two independent experiments. B, coimmunoprecipitation assay, showing association between Rif and Ror1 at endogenous protein levels in the indicated LUAD cells. Whole-cell lysates were subjected to immunoprecipitation (IP) with anti-Ror1 antibody or control immunoglobulin G (isotype matched), followed by Western blotting. Blots are representative of six (PC9), three (HCC827), and two (A549) independent experiments. C and D, representative xz-images of PC9 cells expressing YFP-Rif and Ror1-mCh on a 2D surface ( C ) or Matrigel ( D ), showing colocalization of these proteins at filopodia. Phalloidin staining in ( D ) shows highly accumulated F-actin on the front side containing filopodia. In ( D ), the xy images ( lower panels ) sectioned along the white arrow in xz confocal image ( upper panels ) are shown. Images are representative of at least three independent experiments. The scale bars represent 10 μm. Magnified images of boxed regions are shown on the right . The scale bars in magnified images represent 2 μm ( C ) and 5 μm ( D ). LUAD, lung adenocarcinoma; Rif, Rho in filopodia.

Article Snippet: The short-tandem repeat profiles of PC9, HCC827, and A549 cells were analyzed (BEX CO., LTD), and we confirmed that these cells were not contaminated.

Techniques: Western Blot, Expressing, Co-Immunoprecipitation Assay, Immunoprecipitation, Control, Staining

Journal: International Journal of General Medicine

Article Title: Identification of Prognostic Factors Related to Super Enhancer-Regulated ceRNA Network in Metastatic Lung Adenocarcinoma

doi: 10.2147/IJGM.S332317

Figure Lengend Snippet: Identification of super enhancers in LUAD cells. ( A ) Enhancers were ranked according to the H3K27ac signals in HSC4 and A549 cells based on GSE143653. ( B ) Overlapping analysis of SE-associated genes in HSC4 and A549 cells. ( C ) Functional distribution of the overlapping genes.

Article Snippet: One normal human bronchial epithelial cell line (BEAS-2B) and six LUAD cell lines (H460, HCC827, A549, H1299, PC9 and Calu3) were obtained from American Type Culture Collection (ATCC, VA, USA).

Techniques: Functional Assay

Journal: International Journal of General Medicine

Article Title: Identification of Prognostic Factors Related to Super Enhancer-Regulated ceRNA Network in Metastatic Lung Adenocarcinoma

doi: 10.2147/IJGM.S332317

Figure Lengend Snippet: Effect of AC074117.1 on proliferation of LUAD cells. ( A ) qRT-PCR was used to detect the relative expression of AC074117.1 in BEAS-2B, H460, HCC827, A549, H1299, PC9 and Calu3 cells. ( B ) qRT-PCR was used to measure the knockdown efficiency of AC074117.1 in A549 and H1299 cells. ( C and D ) MTT assay was performed to evaluate changes in cell proliferation following AC074117.1 silencing in A549 and H1299 cells. **P<0.01.

Article Snippet: One normal human bronchial epithelial cell line (BEAS-2B) and six LUAD cell lines (H460, HCC827, A549, H1299, PC9 and Calu3) were obtained from American Type Culture Collection (ATCC, VA, USA).

Techniques: Quantitative RT-PCR, Expressing, Knockdown, MTT Assay